DVL2 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

DVL2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12037-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 cells using 12037-1-AP (same clone as 12037-1-PBS)

  WB result of DVL2 antibody (12037-1-AP, 1:1000) with siDVL2 and si-control transfected cells. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  WB analysis using 12037-1-AP (same clone as 12037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  WB analysis of MDA-MB-453s using 12037-1-AP (same clone as 12037-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 12037-1-AP (same clone as 12037-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12037-1-AP (same clone as 12037-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12037-1-AP (DVL2 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 12037-1-AP (same clone as 12037-1-PBS)

  IP result of anti-DVL2 (IP:12037-1-AP, 4ug; Detection:12037-1-AP 1:4000) with MCF-7 cells lysate 640 ug. This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat colon using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12037-1-AP (same clone as 12037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12037-1-AP (DVL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 12037-1-AP (same clone as 12037-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DVL2 antibody (12037-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12037-1-PBS in a different storage buffer formulation.

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Product Information

12037-1-PBS targets DVL2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DVL2 fusion protein Ag2666
• Full Name: dishevelled, dsh homolog 2 (Drosophila)
• Calculated Molecular Weight: 736 aa, 79 kDa
• Observed Molecular Weight: 90-95 kDa
• GenBank Accession Number: BC014844
• Gene Symbol: DVL2
• Gene ID (NCBI): 1856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DVL2 is member of the dishevelled (dsh) protein family. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein in a variety of cell lines and species. DVL2 participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. It promotes internalization and degradation of frizzled proteins upon Wnt signaling. It plays a role both in canonical and non-canonical Wnt signaling. It also Plays a role in the signal transduction pathways mediated by multiple Wnt genes.