DVL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DVL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

11

Cat no : 27384-1-AP

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Validation Data Gallery

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  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 27384-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 27384-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 27384-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 27384-1-AP (DVL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 27384-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27384-1-AP (DVL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 27384-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using DVL1 antibody (27384-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF-7 cells, NIH/3T3 cells, mouse cerebellum tissue, HEK-293 cells, MDA-MB-231 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue, mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

27384-1-AP targets DVL1 in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DVL1 fusion protein Ag26055
• Full Name: dishevelled, dsh homolog 1 (Drosophila)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 75 kDa, 73 kDa
• GenBank Accession Number: BC050454
• Gene Symbol: DVL1
• Gene ID (NCBI): 1855
• RRID: AB_2880859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

Publications

Species	Application	Title
human	IHC	Theranostics; Transcription factor AP-4 promotes tumorigenic capability and activates the Wnt/β-catenin pathway in hepatocellular carcinoma.; Authors - Junwei Song
mouse	WB	Brain Res Bull; Excess folic acid supplementation before and during pregnancy and lactation activates β-catenin in the brain of male mouse offspring.; Authors - Qian Wu
rat	WB	Neural Plast; Lycopene-Loaded Microemulsion Regulates Neurogenesis in Rats with Aβ-Induced Alzheimer's Disease Rats Based on the Wnt/β-catenin Pathway.; Authors - Wen-Jing Ning
mouse	WB	Biol Trace Elem Res; Selenium Deficiency Leads to Changes in Renal Fibrosis Marker Proteins and Wnt/β-Catenin Signaling Pathway Components.; Authors - Tingting Lin
rat	WB	Neurosci Lett; Dishevelled-1 regulated apoptosis through NF-κB in cerebral ischemia/reperfusion injury in rats.; Authors - Xiaogang Zhang ; KD Validated
human	IHC	J Obstet Gynaecol; cDNA expression library screening revealed novel functional genes involved in clear cell carcinogenesis of the ovary in vitro.; Authors - Yasushi Yamada