DUSP7/PYST2 Polyclonal antibody

DUSP7/PYST2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 26910-1-AP

Synonyms

dual specificity phosphatase 7, DUSP7, MKP X, MKPX, PYST2

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Validation Data Gallery

• 

  WB analysis of rat heart using 26910-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 26910-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26910-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26910-1-AP (DUSP7/PYST2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26910-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26910-1-AP (DUSP7/PYST2 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: rat heart tissue, mouse heart tissue, mouse kidney tissue
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 26910-1-AP is DUSP7/PYST2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP7/PYST2 fusion protein Ag25488
• Full Name: dual specificity phosphatase 7
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: BC104880
• Gene Symbol: DUSP7
• Gene ID (NCBI): 1849
• RRID: AB_2880681
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human dual-specificity phosphatase 7 (DUSP7/PYST2) is a member of a structurally homologous subfamily of MAP kinase phosphatases. DUSP7 is overexpressed in myeloid leukemia and other malignancies. It is known to bind and dephosphorylate ErkII, and as it, along with the other members of the DUSP family expresses high selectively for MAP kinases. It has been suggested that it functions as a method for selectively activating/deactivating different members of that family.

Publications

Species	Application	Title
mouse	WB,IHC	Clin Transl Med; Loss of SETD2 aggravates colorectal cancer progression caused by SMAD4 deletion through the RAS/ERK signalling pathway; Authors - Chunxiao Ma
human	WB	Mol Cancer; Linc-RoR promotes MAPK/ERK signaling and confers estrogen-independent growth of breast cancer.; Authors - Wan-Xin Peng
human	WB	Br J Pharmacol; L-Hcy-induced cathepsin V mediates the vascular endothelial inflammation in hyperhomocysteinemia.; Authors - Yi-Ping Leng
mouse	WB	Addict Biol; Dual-specificity phosphatase 15 (DUSP15) in the nucleus accumbens is a novel negative regulator of morphine-associated contextual memory.; Authors - Xiaomeng Qiao
mouse	WB	Genes Cells; Downregulation of DUSP6, a negative regulator of oncogenic ERK signaling, by ACA-28 induces apoptosis in NIH/3T3 cells overexpressing HER2/ErbB2.; Authors - Yuki Kanda