DUSP16 Polyclonal antibody

DUSP16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 14237-1-AP

Synonyms

DUSP16, KIAA1700, MAP kinase phosphatase 7, MKP 7, MKP7

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Validation Data Gallery

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  WB analysis of PC-12 using 14237-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 14237-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of COLO 320 using 14237-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse brain using 14237-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14237-1-AP (DUSP16 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14237-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14237-1-AP (DUSP16 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: PC-12 cells, COLO 320 cells, HeLa cells, mouse brain tissue
• Positive IHC detected in: human colon tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

14237-1-AP targets DUSP16 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DUSP16 fusion protein Ag5479
• Full Name: dual specificity phosphatase 16
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC042101
• Gene Symbol: DUSP16
• Gene ID (NCBI): 80824
• RRID: AB_2878031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DUSP16, also known as MKP-7, is a mitogen-activated protein kinase phosphatase that is a member of the dual specificity protein phosphatase subfamily. DUSP16 dephosphorylates JUN kinases (JNKs) specifically and thereby regulates T-cell differentiation, which can be responsible for the pathogenesis of Burkitt's lymphoma and cerebral ischemia. DUSP16 was also found to induce the expression of VCAM-1 through the suppression of JNK signaling.

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; NR4A1 enhances MKP7 expression to diminish JNK activation induced by ROS or ER-stress in pancreatic β cells for surviving.; Authors - Ze-Qing Pu
human	WB	Int Immunopharmacol; Berberine derivative DCZ0358 induce oxidative damage by ROS-mediated JNK signaling in DLBCL cells; Authors - Qilin Feng
human,mouse	IHC	J Mol Cell Cardiol; Deficiency of smooth muscle cell ILF3 alleviates intimal hyperplasia via HMGB1 mRNA degradation-mediated regulation of the STAT3/DUSP16 axis; Authors - Ya-Min Hou
human	WB	Int J Hyperthermia; Deregulated JNK signaling enhances apoptosis during hyperthermia; Authors - Atsushi Enomoto

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