DUS2L Recombinant antibody

DUS2L Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240192G8

Cat no : 83265-7-RR

Synonyms

DUS2, DUS2L, FLJ20399, hDUS2, SMM1, URLC8

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Validation Data Gallery

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  WB analysis using 83265-7-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83265-7-RR (DUS2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83265-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83265-7-RR against Human DUS2L were performed. The affinity constant is 1.93 nM.

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Tested Applications

• Positive WB detected in: HepG2 cells, HEK-293 cells, A431 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83265-7-RR is DUS2L Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DUS2L fusion protein Ag23831
• Full Name: dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC006527
• Gene Symbol: DUS2L
• Gene ID (NCBI): 54920
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dihydrouridine synthase 2(DUS2L), is a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. transfection of DUS2 cDNA into a human lung carcinoma cell line increased dihydrouridine in tRNA, whereas transfection of DUS2 small interfering RNA (siRNA) reduced tRNA-dihydrouridine, confirming that DUS2 has dihydrouridine synthase activity.