DTX4 Polyclonal antibody

• Featured Product
• KD/KO Validated

DTX4 Polyclonal Antibody for WB, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 25222-1-AP

Synonyms

Protein deltex 4, KIAA0937, EC:2.3.2.27, E3 ubiquitin-protein ligase DTX4, Deltex4

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Validation Data Gallery

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  WB analysis of mouse kidney using 25222-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 25222-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25222-1-AP (DTX4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse embryo using 25222-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse embryo tissue using 25222-1-AP (DTX4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 25222-1-AP

  Immunofluorescent analysis of HEK-293 cells using 25222-1-AP (DTX4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse kidney tissue
• Positive IF-P detected in: mouse embryo tissue
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

25222-1-AP targets DTX4 in WB, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DTX4 fusion protein Ag18415
• Full Name: deltex homolog 4 (Drosophila)
• Calculated Molecular Weight: 619 aa, 67 kDa
• Observed Molecular Weight: 67-70 kDa
• GenBank Accession Number: BC119011
• Gene Symbol: DTX4
• Gene ID (NCBI): 23220
• RRID: AB_2879970
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DTX4, also named as KIAA0937, RNF155, is a 619 amino acid protein, which belongs to the Deltex family. DTX4 as a regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. DTX4 functions as a ubiquitin ligase protein in vivo, mediating 'Lys48'-linked polyubiquitination and promoting degradation of TBK1, targeting to TBK1 requires interaction with NLRP4.

Publications

Species	Application	Title
human	WB	Cancer Sci; Ubiquitination of NOTCH2 by DTX3 suppresses the proliferation and migration of human esophageal carcinoma.; Authors - Xin-Yu Ding
rat	WB	Stem Cells Dev; Combined Methylome and Transcriptome Analysis During Rat Hepatic Stellate Cell Activation.; Authors - Eva Christine Schumacher
mouse	WB,IF	Biochem Biophys Res Commun; E3 ubiquitin ligase DTX4 is required for adipogenic differentiation in 3T3-L1 preadipocytes cell line.; Authors - Zonggui Wang ; KD Validated
mouse	WB	Cell Death Dis; Gasdermin D silencing alleviates airway inflammation and remodeling in an ovalbumin-induced asthmatic mouse model; Authors - Jinxiang Wu