DSN1 Polyclonal antibody, PBS Only

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  WB analysis of Jurkat using 17742-1-AP (same clone as 17742-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17742-1-AP (DSN1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17742-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 17742-1-AP (same clone as 17742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17742-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 17742-1-AP (same clone as 17742-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17742-1-AP (DSN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17742-1-PBS in a different storage buffer formulation.

DSN1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17742-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

17742-1-PBS targets DSN1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DSN1 fusion protein Ag11988
• Full Name: DSN1, MIND kinetochore complex component, homolog (S. cerevisiae)
• Calculated Molecular Weight: 340 aa, 38 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC026011
• Gene Symbol: DSN1
• Gene ID (NCBI): 79980
• RRID: AB_2878431
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H410
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DSN1, also named as C20orf172 and MIS13, is required to shape an interface between the Mis12 complex and Blinkin, the counterpart of Spc105. DSN1 is part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. DSN1 has four isoforms with MW 27-33 kDa and 38-40 kDa.