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DSG1 Recombinant antibody, PBS Only

DSG1 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241093G11

Cat no : 84053-6-PBS

Synonyms

Desmosomal glycoprotein 1, Desmoglein-1, desmoglein 1, CDHF4, Cadherin family member 4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSG1 Recombinant antibody (84053-6-RR)

    WB analysis using 84053-6-RR (same clone as 84053-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84053-6-RR (DSG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using DSG1 Recombinant antibody (84053-6-RR)

    IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using DSG1 Recombinant antibody (84053-6-RR)

    IHC staining of mouse skin using 84053-6-RR (same clone as 84053-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 84053-6-RR (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84053-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84053-6-RR

    Affinity and Kinetic Characterization of 84053-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84053-6-RR against Human DSG1 were performed. The affinity constant is 71.2 pM.

Product Information

The immunogen of 84053-6-PBS is DSG1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DSG1 fusion protein Ag20184
Full Name desmoglein 1
Calculated Molecular Weight 1049 aa, 114 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC153001
Gene Symbol DSG1
Gene ID (NCBI) 1828
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).