DSG1 Polyclonal antibody

DSG1 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 24587-1-AP

Synonyms

DG1, Desmosomal glycoprotein 1, Desmoglein-1, desmoglein 1, CDHF4

---

Validation Data Gallery

• 

  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 10x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

• 

  IHC staining of human skin cancer using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:400 (under 40x lens). heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

• 

  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 24587-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 24587-1-AP (DSG1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A431 using 24587-1-AP

  Immunofluorescent analysis of A431 cells using 24587-1-AP (DSG1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IHC detected in: human skin cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 9 publications below

Product Information

24587-1-AP targets DSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DSG1 fusion protein Ag20184
• Full Name: desmoglein 1
• Calculated Molecular Weight: 1049 aa, 114 kDa
• GenBank Accession Number: BC153001
• Gene Symbol: DSG1
• Gene ID (NCBI): 1828
• RRID: AB_2879624
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG1 is a single-pass transmembrane glycoprotein highly expressed in the epidermis and localized primarily within the suprabasal epithelial layers (PMID: 16286477; 24220297). DSG1 mediates intercellular adhesion and is crucial in maintaining epidermal integrity and barrier function (PMID: 23974871). It is also involved in epithelial cell differentiation (PMID: 23524961). Mutations in the DSG1 gene can cause the autosomal dominant disorder to striate palmoplantar keratoderma and a syndrome featuring severe dermatitis, multiple allergies, and metabolic wasting (SAM syndrome) (PMID: 29315490; 23974871).

Publications

Species	Application	Title
mouse	WB,IHC	J Nanobiotechnology; ZNPs reduce epidermal mechanical strain resistance by promoting desmosomal cadherin endocytosis via mTORC1-TFEB-BLOC1S3 axis; Authors - Xuan Lai
human	IF	EMBO Rep; O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.; Authors - Ieva Bagdonaite
human	IF	Stem Cell Res Ther; Both Wnt signaling and epidermal stem cell-derived extracellular vesicles are involved in epidermal cell growth.; Authors - Ling Leng
	IF	J Cell Sci; Novel stress granules-like structures are induced via a paracrine mechanism during viral infection.; Authors - Valentina Iadevaia
human	WB,IF	In Vitro Cell Dev Biol Anim; S100A11 is involved in the progression of colorectal cancer through the desmosome-catenin-TCF signaling pathway; Authors - Jin Zhou
mouse	WB,IF	Microscopy (Oxf); Inhibition of retinoid X receptor improved the morphology, localization of desmosomal proteins and paracellular permeability in three-dimensional cultures of mouse keratinocytes.; Authors - Shoko Ishikawa