DSC3 Recombinant antibody, PBS Only

DSC3 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240292B12

Cat no : 83386-5-PBS

Synonyms

DSC1, DSC, Desmocollin 4, desmocollin 3, CDHF3

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Validation Data Gallery

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  WB analysis using 83386-5-RR (same clone as 83386-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83386-5-RR (DSC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 83386-5-RR (same clone as 83386-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83386-5-RR (DSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 83386-5-RR (same clone as 83386-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSC3 antibody (83386-5-RR, Clone: 240292B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83386-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83386-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83386-5-RR against Human DSC3 were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83386-5-PBS is DSC3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DSC3 fusion protein Ag34648
• Full Name: desmocollin 3
• Calculated Molecular Weight: 100 kDa
• GenBank Accession Number: NM_001941
• Gene Symbol: DSC3
• Gene ID (NCBI): 1825
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.