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DSC3 Recombinant antibody

DSC3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240292B12

Cat no : 83386-5-RR

Synonyms

DSC1, DSC, Desmocollin 4, desmocollin 3, CDHF3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DSC3 Recombinant antibody (83386-5-RR)

    WB analysis using 83386-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83386-5-RR (DSC3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse skin tissue using DSC3 Recombinant antibody (83386-5-RR)

    IHC staining of mouse skin using 83386-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 83386-5-RR (DSC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using DSC3 Recombinant antibody (83386-5-RR)

    IF Staining of A431 using 83386-5-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using DSC3 antibody (83386-5-RR, Clone: 240292B12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83386-5-RR

    Affinity and Kinetic Characterization of 83386-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83386-5-RR against Human DSC3 were performed. The affinity constant is below 1 pM.

Tested Applications

Positive WB detected inunboiled A431 cells, A431 cells, HaCaT cells
Positive IHC detected inmouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83386-5-RR is DSC3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen DSC3 fusion protein Ag34648
Full Name desmocollin 3
Calculated Molecular Weight100 kDa
Observed Molecular Weight90-110 kDa
GenBank Accession NumberNM_001941
Gene Symbol DSC3
Gene ID (NCBI) 1825
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Desmocollin-3 (DSC3), also known as CDHF3 and DSC4, is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules and a major component of desmosomes. It has 2 isoforms with molecular masses of 100 and 93 kDa. It is particularly expressed in the vagina, testis, skin, oral mucosa and esophagus. Its subcellular localization is at the cell membrane. Post-translational modifications, including glycosylation, have been described.

Protocols

Product Specific Protocols
WB protocol for DSC3 antibody 83386-5-RRDownload protocol
IHC protocol for DSC3 antibody 83386-5-RRDownload protocol
IF protocol for DSC3 antibody 83386-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols