DRP1 (C-terminal) Recombinant antibody, PBS Only

• Featured Product
• KD/KO Validated

DRP1 (C-terminal) Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A19

Cat no : 81561-1-PBS

Synonyms

DRP1, Dnm1p/Vps1p-like protein, DNM1L,DLP1, DNM1L, DLP1

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Validation Data Gallery

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  WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 81561-1-RR (same clone as 81561-1-PBS)

  WB result of DRP1 (C-terminal) antibody (81561-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DRP1 (C-terminal) transfected HEK-293 cells. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 81561-1-RR (same clone as 81561-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 81561-1-RR (same clone as 81561-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 81561-1-RR (DRP1 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 81561-1-RR (same clone as 81561-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using DRP1 (C-terminal) antibody (81561-1-RR, Clone: 2A19 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 81561-1-RR (same clone as 81561-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DRP1 (C-terminal) (81561-1-RR, Clone:2A19) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81561-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81561-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81561-1-RR against Human DRP1 were performed. The affinity constant is below 1 pM.

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Product Information

81561-1-PBS targets DRP1 (C-terminal) in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DRP1 (C-terminal) fusion protein Ag3644
• Full Name: dynamin 1-like
• Calculated Molecular Weight: 736 aa, 82 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC024590
• Gene Symbol: DRP1
• Gene ID (NCBI): 10059
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

DNM1L(Dynamin-1-like protein) , also known as dynamin-related protein 1 (DRP1), belongs to the dynamin family of large GTPases that mediate membrane remodeling during a variety of cellular processes. DNM1L has an important role in the division of growing mitochondria and peroxisomes and also mediates outer mitochondrial membrane fission in mammalian cells. DNM1L is ubiquitously expressed with abundant expression in skeletal muscle, heart, kidney and brain.Variable isoforms of DNM1L have been reported in a tissue-specific manner due to the alternative splicing.