DROSHA Polyclonal antibody, PBS Only

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  WB analysis using 27958-1-AP (same clone as 27958-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27958-1-AP (DROSHA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 27958-1-AP (same clone as 27958-1-PBS)

  WB result of DROSHA antibody (27958-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DROSHA transfected HEK-293 cells. This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 27958-1-AP (same clone as 27958-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DROSHA antibody (27958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 27958-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DROSHA Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 27958-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

RNASEN, EC:3.1.26.3, ETOHI2, HSA242976, p241

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Product Information

27958-1-PBS targets DROSHA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DROSHA fusion protein Ag27533
• Full Name: ribonuclease type III, nuclear
• Calculated Molecular Weight: 159 aa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: NM_001100412
• Gene Symbol: DROSHA
• Gene ID (NCBI): 29102
• RRID: AB_2918137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NRR4
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DROSHA, also named as RN3, RNASE3L, P241 and RNASEN, is double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. It is a component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. RNASEN is involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. And it is involved in the formation of GW bodies. The antibody is specific to DROSHA.