DRG2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DRG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 14743-1-AP

Synonyms

Translation factor GTPase DRG2, TRAFAC GTPase DRG2, EC:3.6.5.-, DRG-2, DRG 2

---

Validation Data Gallery

• 

  WB analysis using 14743-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14743-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14743-1-AP (DRG2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14743-1-AP

  IP result of anti-DRG2 (IP:14743-1-AP, 4ug; Detection:14743-1-AP 1:2000) with mouse brain tissue lysate 2640ug.

• 

  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 14743-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14743-1-AP (DRG2 antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 14743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DRG2 antibody (14743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, SMMC-7721 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:3000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

14743-1-AP targets DRG2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRG2 fusion protein Ag6433
• Full Name: developmentally regulated GTP binding protein 2
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 41-45 kDa
• GenBank Accession Number: BC000493
• Gene Symbol: DRG2
• Gene ID (NCBI): 1819
• RRID: AB_2230800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Developmentally regulated GTP-binding protein (DRG) is a new subfamily within the superfamily of GTP-binding proteins. Its expression is regulated during embryonic development. DRG2 plays a critical role in control of the cell cycle and apoptosis (PMID:27669826, PMID:14759600). DRG2 Regulates G2/M Progression via the Cyclin B1-Cdk1 Complex.

Publications

Species	Application	Title
rat	WB	Mol Ther Nucleic Acids; Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.; Authors - Zhijie Shen
human	WB	Int J Mol Sci; DRG2 Depletion Promotes Endothelial Cell Senescence and Vascular Endothelial Dysfunction.; Authors - Anh-Nhung Le ; KO Validated
human	WB,IF,IP	Oxid Med Cell Longev; DRG2 Accelerates Senescence via Negative Regulation of SIRT1 in Human Diploid Fibroblasts; Authors - Bing Si Li ; KD Validated
mouse	IF	FASEB J; Global analyses of mRNA expression in human sensory neurons reveal eIF5A as a conserved target for inflammatory pain.; Authors - Rebecca Chase
human,monkey	WB	Mol Cell Endocrinol; RWDD1 interacts with the ligand binding domain of the androgen receptor and acts as a coactivator of androgen-dependent transactivation.; Authors - Gr?tsch Helga H
human	WB	Biochem Biophys Res Commun; Developmentally regulated GTP-binding protein 2 depletion leads to mitochondrial dysfunction through downregulation of dynamin-related protein 1.; Authors - Mai-Tram Vo ; KD Validated