DRC1 Polyclonal antibody

DRC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26219-1-AP

Synonyms

C2orf39, Dynein regulatory complex protein 1, Coiled-coil domain-containing protein 164, CCDC164

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Validation Data Gallery

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  WB analysis using 26219-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26219-1-AP (DRC1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of hTERT-RPE1 using 26219-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DRC1 antibody (26219-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CoraLite®594 ARL13B antibody (CL594-17711, red).

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IF/ICC detected in: hTERT-RPE1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26219-1-AP targets DRC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DRC1 fusion protein Ag24113
• Full Name: chromosome 2 open reading frame 39
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC009488
• Gene Symbol: C2orf39/DRC1
• Gene ID (NCBI): 92749
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DRC1, also known as C2orf39, CCDC164, has 3 coiled-coil motifs in its N-terminal half and an additional coiled-coil motif near its C terminus. DRC1 is a subunit of the nexin-dynein regulatory complex, an axonemal structure critical for the regulation of ciliary dynein motors. DRC1 is predominantly expressed in the lung, brain, and prostate (PMID: 23354437). DRC1 deficiency disrupts the assembly of the nexin-dynein regulatory complex, causing ciliary dyskinesia(PMID: 23354437, 34815526).