DR5 Recombinant antibody

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  WB analysis of NIH/3T3 using 84524-4-RR

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84524-4-RR (DR5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DR5 Recombinant Antibody for WB, ELISA

Cat No. 84524-4-RR

Clone No.241847B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Tnfrsf10b, MK, Killer, Death receptor 5, CD262

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Tested Applications

• Positive WB detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84524-4-RR targets DR5 in WB, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: tumor necrosis factor receptor superfamily, member 10b
• Calculated Molecular Weight: 42kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: NM_020275.4
• Gene Symbol: DR5
• Gene ID (NCBI): 21933
• RRID: AB_3672032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9QZM4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DR5, also known as CD262, TNFRSF10B, TRAILR2, TRICK2 and KILLER, is a widely expressed single-pass type I membrane protein belonging to the tumour necrosis factor receptor superfamily (TNFRSF). It is a receptor for TNF-related apoptosis-inducing ligand (TRAIL), which is a member of the tumor necrosis factor (TNF) family of cytokines and induces apoptosis in a wide variety of cells (PMID: 9311998). DR5 contains two extracellular cysteine-rich repeats, typical for TNF receptor (TNFR) family members, and a cytoplasmic death domain (DD), through which DR5 is capable to transmit the apoptotic signal (PMID: 9311998; 20531300).