CRMP5 Polyclonal antibody

CRMP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10525-1-AP

Synonyms

DPYSL5, DPYSL 5, DPYS L5, Dihydropyrimidinase-related protein 5, CRMP-5

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Validation Data Gallery

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  WB analysis using 10525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10525-1-AP (CRMP5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10525-1-AP

  IP result of anti-CRMP5 (IP:10525-1-AP, 4ug; Detection:10525-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 10525-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10525-1-AP (CRMP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 10525-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRMP5 antibody (10525-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

10525-1-AP targets CRMP5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CRMP5 fusion protein Ag0783
• Full Name: dihydropyrimidinase-like 5
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC002874
• Gene Symbol: CRMP5
• Gene ID (NCBI): 56896
• RRID: AB_2094644
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPYSL5(Dihydropyrimidinase-related protein 5) is also named as CRMP5, ULIP6 and belongs to the hydantoinase/dihydropyrimidinase subfamily. It may have a function in neuronal differentiation and/or axon growth. SCLCs universally express CRMP5 and in thymoma, none of the CRMPs are detected by immunohistochemistry in tumorous epithelial cells with specific antibodies including CRMP5.The normal CRMP5 is probably not expressed by tumorous epithelial cells.(PMID:16519949).

Publications

Species	Application	Title
mouse	WB	J Proteomics; Network of brain protein level changes in glutaminase deficient fetal mice.; Authors - Bae Narkhyun N
human	WB,IHC	J Int Med Res; Ubiquitin C-terminal hydrolase L1 promotes lymph node metastasis in small cell neuroendocrine carcinomas of the cervix.; Authors - Yunqiang Zhang