DPYD Polyclonal antibody

DPYD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 27662-1-AP

Synonyms

DHP, DHPDHASE, Dihydrothymine dehydrogenase, Dihydrouracil dehydrogenase, DPD, DPYD

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Validation Data Gallery

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  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 27662-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 27662-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 27662-1-AP (DPYD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 27662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27662-1-AP (DPYD antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: rat liver tissue, L02 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

27662-1-AP targets DPYD in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPYD fusion protein Ag26599
• Full Name: dihydropyrimidine dehydrogenase
• Calculated Molecular Weight: 1025 aa, 111 kDa
• Observed Molecular Weight: 111 kDa
• GenBank Accession Number: BC008379
• Gene Symbol: DPYD
• Gene ID (NCBI): 1806
• RRID: AB_2880941
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Pharmgenomics Pers Med; Exploration and Validation of Pancreatic Cancer Hub Genes Based on Weighted Gene Co-Expression Network Analysis and Immune Infiltration Score Analysis; Authors - Xiao-Xi Li
human	IHC	J Cell Mol Med; Pathway-based stratification of gliomas uncovers four subtypes with different TME characteristics and prognosis; Authors - Ruoyu Huang
human,mouse	IF	Theranostics; Single cell analyses reveal the PD-1 blockade response-related immune features in hepatocellular carcinoma; Authors - Yao Li
human	WB	J Clin Invest; CIAO1 loss of function causes a neuromuscular disorder with compromise of nucleocytoplasmic Fe-S enzymes; Authors - Nunziata Maio