DPP6 Polyclonal antibody

DPP6 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 28143-1-AP

Synonyms

DPP VI, Dipeptidyl peptidase VI, Dipeptidyl peptidase IV-like protein, Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-related protein

---

Validation Data Gallery

• 

  WB analysis using 28143-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28143-1-AP (DPP6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 28143-1-AP

  IP result of anti-DPP6 (IP:28143-1-AP, 4ug; Detection:28143-1-AP 1:4000) with mouse brain tissue lysate 1840 ug.

• 

  IF Staining of SH-SY5Y using 28143-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DPP6 antibody (28143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28143-1-AP targets DPP6 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPP6 fusion protein Ag27338
• Full Name: dipeptidyl-peptidase 6
• Observed Molecular Weight: 98-101 kDa
• GenBank Accession Number: BC150304
• Gene Symbol: DPP6
• Gene ID (NCBI): 1804
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP6 (Dipeptidyl aminopeptidase-like protein 6) is also named as DPPX and Dipeptidyl peptidase VI (DPP VI). DPP6 is a type II transmembrane protein, and DPP6 is a multifunctional protein with considerable impact on brain development and function (PMID: 35395074). In breast cancer tissues, DPP6 has low expression at the transcription and protein levels, and DPP6 may serve as a tumour suppressor in tumour development (PMID: 36531033).