DPP4/CD26 Monoclonal antibody, PBS Only (Capture)

DPP4/CD26 Monoclonal Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6D11

Cat no : 68383-1-PBS

Synonyms

CD26, ADCP-2, ADCP2, ADCP 2, ADABP

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Validation Data Gallery

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  WB analysis of human placenta using 68383-1-Ig (same clone as 68383-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig kidney using 68383-1-Ig (same clone as 68383-1-PBS)

  pig kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit kidney using 68383-1-Ig (same clone as 68383-1-PBS)

  rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68383-1-Ig (DPP4/CD26 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA standard curve of MP50051-1

  Sandwich ELISA standard curve of MP50051-1, DPP4 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68383-1-PBS. Detection antibody: 68383-2-PBS. Standard: Eg0157. Range: 0.313-20 ng/mL.

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  IF Staining of HuH-7 using 68383-1-Ig (same clone as 68383-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HuH-7 cells using DPP4/CD26 antibody (68383-1-Ig, Clone: 2G6D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68383-1-PBS in a different storage buffer formulation.

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Product Information

68383-1-PBS targets DPP4/CD26 as part of a matched antibody pair:

MP50051-1: 68383-1-PBS capture and 68383-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 110 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).