DPP4/CD26 Polyclonal antibody

• Featured Product
• KD/KO Validated

DPP4/CD26 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10940-1-AP

Synonyms

ADABP, ADCP 2, ADCP2, CD26, dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, DPP4, DPPIV, T cell activation antigen CD26, TP103

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Validation Data Gallery

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  WB analysis of PC-3 using 10940-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10940-1-AP (DPP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10940-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10940-1-AP (DPP4 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of PC-3 using 10940-1-AP

  Immunofluorescent analysis of PC-3 cells using 10940-1-AP (DPP4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

10940-1-AP targets DPP4/CD26 in WB, IF, IHC, ELISA applications and shows reactivity with human, hamster samples.

• Tested Reactivity: human, hamster
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DPP4/CD26 fusion protein Ag1380
• Full Name: dipeptidyl-peptidase 4
• Calculated Molecular Weight: 88 kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC013329
• Gene Symbol: DPP4
• Gene ID (NCBI): 1803
• RRID: AB_2093567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DPP4 (also known as CD26) is a serine exopeptidase that cleaves X-proline dipeptides from the N terminus of polypeptides. It is an intrinsic membrane glycoprotein anchored into the cell membrane by its N-terminal end. High levels of the enzyme are found in the brush-border membranes of the kidney proximal tubule and of the small intestine, but several other tissues also express the enzyme. The enzyme is present in the fetal colon but disappears at birth. It is ectopically expressed in some human colon adenocarcinomas and human colon cancer cell lines(PMID:1977364).The dimeric 150- 220 kDa DPPIV has been reported to be active and accessible to DFP labeling, but the 110 kDa monomeric DPPIV is not(PMID:9065413). Sometimes traces of the 290 kDa active dimeric form of DPP IV as well as a 55-60 kDa protein appeared in the immunopurified DPP IV preparation. N-terminal amino acid sequence analysis revealed that the 55-60 kDa protein represents a fragment of DPP IV starting at amino acid 28.(PMID:9654125).

Publications

Species	Application	Title
human	WB	Oncogene; ApoC1 promotes the metastasis of clear cell renal cell carcinoma via activation of STAT3.; Authors - Yang-Ling Li
rat	WB	Hypertension; Dipeptidyl peptidase IV regulates proliferation of preglomerular vascular smooth muscle and mesangial cells.; Authors - Jackson Edwin K EK
human	WB	Int J Mol Sci; Preclinical Repurposing of Sitagliptin as a Drug Candidate for Colorectal Cancer by Targeting CD24/CTNNB1/SOX4-Centered Signaling Hub; Authors - Jing-Wen Shih
human,rat	WB,IF,IHC	Biochem Pharmacol; Dipeptidyl peptidase-4 inhibition improves endothelial senescence by activating AMPK/SIRT1/Nrf2 signaling pathway.; Authors - Zhihui Chen ; KD Validated
rat	WB	Endocrinology; Dipeptidylpeptidase inhibition is associated with improvement in blood pressure and diastolic function in insulin-resistant male Zucker obese rats.; Authors - Aroor Annayya R AR
rat	IF	J Agric Food Chem; A Low ω-6/ω-3 Ratio High-Fat Diet Improves Rat Metabolism via Purine and Tryptophan Metabolism in the Intestinal Tract, While Reversed by Inulin.; Authors - Yao Xiao