DOK1 Polyclonal antibody

DOK1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17822-1-AP

Synonyms

pp62, P62DOK, Docking protein 1

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Validation Data Gallery

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  WB analysis of K-562 using 17822-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17822-1-AP (DOK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 17822-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17822-1-AP (DOK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 17822-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17822-1-AP (DOK1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17822-1-AP targets DOK1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOK1 fusion protein Ag12189
• Full Name: docking protein 1, 62kDa (downstream of tyrosine kinase 1)
• Calculated Molecular Weight: 48 aa, 52 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC111055
• Gene Symbol: DOK1
• Gene ID (NCBI): 1796
• RRID: AB_2274420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The downstream of tyrosine kinase 1gene (DOK1) has emerged as a newly identified tumor suppressor gene that encodes a multi-domain adapter protein and acts as a negative regulator of signaling pathways involved in several cellular functions. DOK1 inhibits cell proliferation, down regulates MAP kinase activity, and has an opposing role in leukemogenesis and promotes cell spreading, motility, and apoptosis. (PMID: 24809689)