DOHH Monoclonal antibody, PBS Only

DOHH Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H5H7

Cat no : 68151-1-PBS

Synonyms

Deoxyhypusine dioxygenase, Deoxyhypusine hydroxylase, Deoxyhypusine monooxygenase, DOHH, hDOHH, HLRC1

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Validation Data Gallery

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  WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68151-1-Ig (same clone as 68151-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68151-1-Ig (DOHH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68151-1-PBS in a different storage buffer formulation.

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Product Information

68151-1-PBS targets DOHH in WB, Indirect ELISA applications and shows reactivity with Human, Rat, Mouse samples.

• Tested Reactivity: Human, Rat, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DOHH fusion protein Ag13041
• Full Name: deoxyhypusine hydroxylase/monooxygenase
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC002817
• Gene Symbol: DOHH
• Gene ID (NCBI): 83475
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Deoxyhypusine hydroxylase (DOHH) is the enzyme catalyzing the second step in the post-translational synthesis of hypusine [Nε-(4-amino-2-hydroxybutyl)lysine] in the eukaryotic initiation factor 5A (eIF5A). DOHH has been reported to mediate several crucial cellular functions, including cellular proliferation, differentiation and apoptosis. Moreover, previous studies have established that DOHH is highly involved in several essential biological processes driving human diseases including cancer growth, malarial drug resistance, and HIV-1 replication. In particular, the DOHH/eIF5A signaling pathway was revealed to mediate the ability of nerve growth factor to enhance neuronal growth and survival, highlighting the importance of DOHH in neuroprotection(PMID: 35007708, PMID: 16371467, PMID: 22908221, PMID: 19706422).