DOCK7 Polyclonal antibody

DOCK7 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13000-1-AP

Synonyms

ZIR2, dedicator of cytokinesis 7

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Validation Data Gallery

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  WB analysis using 13000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13000-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 13000-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13000-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13000-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13000-1-AP (DOCK7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13000-1-AP

  IP result of anti-DOCK7 (IP:13000-1-AP, 4ug; Detection:13000-1-AP 1:10000) with HeLa cells lysate 1280 ug.

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  IP experiment of HeLa using 13000-1-AP

  IP result of anti-DOCK7 (IP:13000-1-AP, 4ug; Detection:13000-1-AP 1:1000) with HeLa cells lysate 2640 ug.

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  IF Staining of HeLa using 13000-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DOCK7 antibody (13000-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293T cells, mouse brain tissue, human brain tissue, mouse ovary tissue, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13000-1-AP targets DOCK7 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOCK7 fusion protein Ag3671
• Full Name: dedicator of cytokinesis 7
• Calculated Molecular Weight: 2109 aa, 239 kDa
• Observed Molecular Weight: 238-243 kDa
• GenBank Accession Number: BC016392
• Gene Symbol: DOCK7
• Gene ID (NCBI): 85440
• RRID: AB_10646476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK 7 (dedicator of cytokinesis 7), also known as ZIR2, is a member of the DOCK180-related protein superfamily. Expressed mainly in neuronal cells, DOCK 7 is a guanine nucleotide exchange factor (GEF) for small GTPases, Rac1 and Cdc42, which are the major regulators of actin cytoskeleton. Multiple isoforms of DOCK 7 exist due to alternative splicing events. The DOCK 7 antibody (13000-1-AP) can recognize all the isoforms.

Publications

Species	Application	Title
mouse	WB,IF	Clin Transl Med; Tumour-associated macrophage-derived DOCK7-enriched extracellular vesicles drive tumour metastasis in colorectal cancer via the RAC1/ABCA1 axis; Authors - Weiwei Chen ; KD Validated
human	WB	Mol Biol Cell; The WASH complex, an endosomal Arp2/3 activator, interacts with the Hermansky-Pudlak syndrome complex BLOC-1 and its cargo phosphatidylinositol-4-kinase type IIα.; Authors - P V Ryder
mouse	WB	Nat Cell Biol; EGFR ligand shifts the role of EGFR from oncogene to tumour suppressor in EGFR-amplified glioblastoma by suppressing invasion through BIN3 upregulation.; Authors - Gao Guo
human	WB,IF,IP,CoIP	Neurobiol Dis; The Warburg micro syndrome protein RAB3GAP1 modulates neuronal morphogenesis and interacts with axon elongation end ER-Golgi trafficking factors; Authors - Pankaj S Ghate
human	WB	Phytomedicine; Protective effect of ethyl ferulate against hypoxic injury in retinal cells and retinal neovascularization in an oxygen-induced retinopathy model; Authors - Rong Xue