DOCK2-Specific Polyclonal antibody

DOCK2-Specific Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20245-1-AP

Synonyms

dedicator of cytokinesis 2, DOCK2, DOCK2-Specific, KIAA0209

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Validation Data Gallery

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  WB analysis using 20245-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20245-1-AP (DOCK2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 20245-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DOCK2-Specific antibody (20245-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, Ramos cells
• Positive IF/ICC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20245-1-AP targets DOCK2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: dedicator of cytokinesis 2
• Calculated Molecular Weight: 212 kDa
• Observed Molecular Weight: 212 kDa
• GenBank Accession Number: NM_004946
• Gene Symbol: DOCK2
• Gene ID (NCBI): 1794
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOCK2, also named as KIAA0209, belongs to the DOCK family. It is involved in cytoskeletal rearrangements required for lymphocyte migration in response of chemokines. DOCK2 activates RAC1 and RAC2 small GTPases, probably by functioning as a guanine nucleotide exchange factor (GEF), which exchanges bound GDP for free GTP. DOCK2 may also participate in IL2 transcriptional activation via the activation of RAC2. The antibody is specific to DOCK2.