DOCK180 Polyclonal antibody, PBS Only

DOCK180 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23421-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293 using 23421-1-AP (same clone as 23421-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 23421-1-AP (same clone as 23421-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 23421-1-AP (same clone as 23421-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 23421-1-AP (same clone as 23421-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23421-1-AP (DOCK180 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

  IP result of anti-DOCK180 (IP:23421-1-AP, 4ug; Detection:23421-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 23421-1-AP (same clone as 23421-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon slide using 23421-1-AP (DOCK180 antibody at dilution of 1:50. This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23421-1-AP (same clone as 23421-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 23421-1-AP (DOCK180 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23421-1-PBS in a different storage buffer formulation.

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Product Information

23421-1-PBS targets DOCK180 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOCK180 fusion protein Ag18069
• Full Name: dedicator of cytokinesis 1
• Calculated Molecular Weight: 1865 aa, 215 kDa
• Observed Molecular Weight: 215 kDa
• GenBank Accession Number: BC146857
• Gene Symbol: DOCK1
• Gene ID (NCBI): 1793
• RRID: AB_2879276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.