DOC2A Polyclonal antibody

DOC2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20575-1-AP

Synonyms

Doc2, Doc2 alpha, DOC2A, double C2 like domains, alpha

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Validation Data Gallery

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  WB analysis using 20575-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20575-1-AP (DOC2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20575-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20575-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20575-1-AP (DOC2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20575-1-AP targets DOC2A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: double C2-like domains, alpha
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 18-44 kDa
• GenBank Accession Number: NM_003586
• Gene Symbol: DOC2A
• Gene ID (NCBI): 8448
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DOC2, Double C2-like domain-containing protein alpha, is involved in Ca(2+)-dependent neurotransmitter release. DOC2A and DOC2B are sensors for neuronal activity with unique calcium-dependent and kinetic properties (PMID: 16515538). DOC2A is mainly expressed in brain and also expressed in testis (PMID: 7826360).