DNase I Polyclonal antibody, PBS Only

• 

  WB analysis using 16659-1-AP (same clone as 16659-1-PBS)

  human urine sample were subjected to SDS PAGE followed by western blot with 16659-1-AP (DNase I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16659-1-PBS in a different storage buffer formulation.

DNase I Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16659-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

---

Product Information

16659-1-PBS targets DNase I in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNase I fusion protein Ag10117
• Full Name: deoxyribonuclease I
• Calculated Molecular Weight: 282 aa, 31 kDa
• Observed Molecular Weight: 31-40 kDa
• GenBank Accession Number: BC029437
• Gene Symbol: DNASE1
• Gene ID (NCBI): 1773
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P24855
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).