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DNase I Polyclonal antibody, PBS Only

DNase I Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16659-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using DNase I Polyclonal antibody (16659-1-AP)

    WB analysis using 16659-1-AP (same clone as 16659-1-PBS)

    human urine sample were subjected to SDS PAGE followed by western blot with 16659-1-AP (DNase I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16659-1-PBS in a different storage buffer formulation.

Product Information

16659-1-PBS targets DNase I in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DNase I fusion protein Ag10117
Full Name deoxyribonuclease I
Calculated Molecular Weight 282 aa, 31 kDa
Observed Molecular Weight 31-40 kDa
GenBank Accession NumberBC029437
Gene Symbol DNASE1
Gene ID (NCBI) 1773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).