Validation Data Gallery
|Positive WB detected in||Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cels, mouse testis tissue, rat testis tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human stomach cancer tissue, mouse colon tissue, mouse testis tissue, rat colon tissue, rat testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 20954-1-AP is DNMT3A Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DNMT3A fusion protein Ag15111|
|Full Name||DNA (cytosine-5-)-methyltransferase 3 alpha|
|Calculated molecular weight||912 aa, 102 kDa|
|Observed molecular weight||120-130 kDa|
|GenBank accession number||BC043617|
|Gene ID (NCBI)||1788|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.
Coeloglossum viride var. bracteatum extract attenuates staurosporine induced neurotoxicity by restoring the FGF2-PI3K/Akt signaling axis and Dnmt3.
Cell Biol Int
miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.
Res Vet Sci
Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.
Integr Cancer Ther
Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.
DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.
Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1β-Induced Osteoarthritis Progression Through Regulating DNMT3A.