Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HepG2 cells, HeLa cells, NIH/3T3 cels, mouse testis tissue, rat testis tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, mouse colon tissue, rat colon tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20954-1-AP targets DNMT3A in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15111 Product name: Recombinant human DNMT3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC043617 Sequence: MPAMPSSGPGDTSSSAAEREEDRKDGEEQEEPRGKEERQEPSTTARKVGRPGRKRKHPPVESGDTPKDPAVISKSPSMAQDSGASELLPNGDLEKRSEPQPEEGSPAGGQKGGAPAEGEGAAETLPEASRAVENGCCTPKEGRGAPAEAGKEQKETNIESMKMEGSRGRLRGGLGWESSLRQRPMPRLTFQAGDPYYISKRKRDEWLARWKREAEKKAKVIAGMNAVEENQGPGESQKVEEASPPAVQQPTDPASPTVATTPEPVGSDAGDKNATKAGDD Predict reactive species |
| Full Name | DNA (cytosine-5-)-methyltransferase 3 alpha |
| Calculated Molecular Weight | 912 aa, 102 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC043617 |
| Gene Symbol | DNMT3A |
| Gene ID (NCBI) | 1788 |
| RRID | AB_10733339 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y6K1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNA methylation in vertebrate animals is an epigenetic modification that is important for embryonic development, imprinting, and the inactivation of X chromosomes. DNA methylation is catalyzed by a family of DNA methyltransferases (DNMTs) that include the maintenance enzyme DNMT1 and de novo methyltransferases DNMT3a and DNMT3b. The overexpression of DNMT1, DNMT3a, and DNMT3b has been reported in various malignancies, including gastric, urothelial, and lung cancers, and may be related to tumorigenesis, tumor progression, and poor survival. Two isoforms of DNMT3a exist: the full-length DNMT3a, and the shorter form DNMT3a2 which lacks the N-terminal fragment. DNMT3a is expressed ubiquitously at low levels, while DNMT3a2 is specially expressed at high levels in embryonic stem cells and shows restricted expression in tissues known to undergo de novo methylation including testis and ovary. This antibody was raised against the N-terminal region of human DNMT3a. It is expected to detect the 120-130 kDa DNMT3a but not 72-100 kDa DNMT3a2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DNMT3A antibody 20954-1-AP | Download protocol |
| IHC protocol for DNMT3A antibody 20954-1-AP | Download protocol |
| IP protocol for DNMT3A antibody 20954-1-AP | Download protocol |
| WB protocol for DNMT3A antibody 20954-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNMT3A Polyclonal antibody (Cat# 20954-1-AP) has 83 citations in journals including Nature Communications, Molecular Cancer, Science Advances, Advanced Science, Cell Reports Medicine, Biomaterials, Genome Biology, and Aging Cell. Research fields encompass epigenetics/DNA methylation, oncology (lung, breast, colorectal, gastric, ovarian, glioma), cardiology, neuroscience, immunology, developmental biology, fibrosis, and inflammation. Cited applications include WB, IHC, IF, IP, CoIP, ChIP, and RIP.
| Species | Application | Title |
|---|---|---|
Mol Cancer CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug
| ||
Protein Cell Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells. | ||
Nat Commun Loss of DNA methyltransferase 3a enhances Caspase-8 transcription and promotes cardiomyocyte pyroptosis during myocardial infarction. | ||
Nat Commun Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation | ||
J Adv Res UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis. |
