DNMT1 Polyclonal antibody

DNMT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28754-1-AP

Synonyms

EC:2.1.1.37, DNMT, DNA (cytosine-5)-methyltransferase 1, CXXC-type zinc finger protein 9, CXXC9

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Validation Data Gallery

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  WB analysis using 28754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28754-1-AP (DNMT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293T cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28754-1-AP targets DNMT1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNMT1 fusion protein Ag29479
• Full Name: DNA (cytosine-5-)-methyltransferase 1
• Calculated Molecular Weight: 1632 aa, 185 kDa
• Observed Molecular Weight: 145 kDa and 184 kDa
• GenBank Accession Number: BC126227
• Gene Symbol: DNMT1
• Gene ID (NCBI): 1786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA methylation is a major epigenetic modification that regulates gene expression. DNMT1, the maintenance DNA methylation enzyme, is the primary enzyme responsible for copying methylation patterns after DNA replication. DNMT1 is required for X chromosome inactivation, imprinting, genomic methylation and proper embryonic development. Overexpression of DNMT1 has been reported in various cancers. DNMT1 exists some isoforms with the molecular mass of 184 kDa and 145 kDa. (PMID: 10753866)