DNM3 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNM3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14737-1-AP

Synonyms

T-dynamin, T dynamin, KIAA0820, EC:3.6.5.5, Dynamin-3

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Validation Data Gallery

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  WB analysis using 14737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14737-1-AP (DNM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14737-1-AP (DNM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14737-1-AP

  IP result of anti-DNM3 (IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 14737-1-AP

  IP result of anti-DNM3 (IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14737-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM3 antibody (14737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, fetal human brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Product Information

14737-1-AP targets DNM3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNM3 fusion protein Ag6381
• Full Name: dynamin 3
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 97-100 kDa
• GenBank Accession Number: BC064546
• Gene Symbol: DNM3
• Gene ID (NCBI): 26052
• RRID: AB_2093691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)

Publications

Species	Application	Title
	IF	Mol Cell Proteomics; Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals; Authors - Brett Nixon
mouse	IF	Mol Cell Proteomics; Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.; Authors - Brett Nixon
mouse	WB,IHC,IF	J Biol Chem; Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.; Authors - Reid Andrew T AT
human	IF	Mol Hum Reprod; Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.; Authors - Wei Zhou
human	WB	Clin Exp Metastasis; Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.; Authors - Natascia Marino ; KD Validated
mouse	WB,IF	Biol Reprod; Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis.; Authors - Wei Zhou