DNM2 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 68209-1-Ig (same clone as 68209-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68209-1-Ig (same clone as 68209-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50954-1

  Cytometric bead array standard curve of MP50954-1, DNM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68209-1-PBS. Detection antibody: 68209-2-PBS. Standard:Ag6274. Range: 0.781-100 ng/mL.

DNM2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 68209-1-PBS

Clone No.1B8H3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

dynamin2, Dynamin II, dynamin 2, DYN2, DNM 2

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Product Information

68209-1-PBS targets DNM2 as part of a matched antibody pair:

MP50954-1: 68209-1-PBS capture and 68209-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNM2 fusion protein Ag6274
• Full Name: dynamin 2
• Calculated Molecular Weight: 98 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC054501
• Gene Symbol: DNM2
• Gene ID (NCBI): 1785
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50570
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.