DNASE2B Polyclonal antibody

DNASE2B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 22652-1-AP

Synonyms

DNase II like acid DNase, DNase II beta, DLAD, Deoxyribonuclease-2-beta, Deoxyribonuclease II beta

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Validation Data Gallery

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  WB analysis of Jurkat using 22652-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 22652-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22652-1-AP (DNASE2B Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 22652-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 22652-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 22652-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 22652-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22652-1-AP (DNASE2B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of A549 using 22652-1-AP

  Immunofluorescent analysis of A549 cells using 22652-1-AP (DNASE2B antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, A549 cells
• Positive IHC detected in: human tonsillitis tissue, human prostate hyperplasia tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22652-1-AP targets DNASE2B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNASE2B fusion protein Ag18246
• Full Name: deoxyribonuclease II beta
• Calculated Molecular Weight: 361 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC126136
• Gene Symbol: DNASE2B
• Gene ID (NCBI): 58511
• RRID: AB_2879144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Int J Biochem Cell Biol; Heat shock factor 4 regulates lens epithelial cell homeostasis by working with lysosome and anti-apoptosis pathways.; Authors - Xiukun Cui