DNASE2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNASE2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15934-1-AP

Synonyms

DNASE2A, DNase II alpha, Deoxyribonuclease-2-alpha, Deoxyribonuclease II alpha, Deoxyribonuclease 2 alpha

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Validation Data Gallery

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  WB analysis of HeLa using 15934-1-AP

  WB result of DNASE2 antibody (15934-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNASE2 transfected HeLa cells.

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 15934-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15934-1-AP (DNASE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of U-87 MG using 15934-1-AP

  IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with U-87 MG cells lysate 1160 ug.

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  IP experiment of HeLa using 15934-1-AP

  IP result of anti-DNASE2 (IP:15934-1-AP, 4ug; Detection:15934-1-AP 1:500) with HeLa cells lysate 1360 ug.

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15934-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15934-1-AP (DNASE2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 15934-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNASE2 antibody (15934-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, LNCaP cells, Jurkat cells, U-87 MG cells, MDA-MB-231 cells
• Positive IP detected in: U-87 MG cells, HeLa cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

15934-1-AP targets DNASE2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNASE2 fusion protein Ag8781
• Full Name: deoxyribonuclease II, lysosomal
• Calculated Molecular Weight: 360 aa, 40 kDa
• Observed Molecular Weight: 32-40 kDa
• GenBank Accession Number: BC010419
• Gene Symbol: DNASE2
• Gene ID (NCBI): 1777
• RRID: AB_2095546
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNASE2, or 'acid DNase,' hydrolyzes DNA under acidic conditions and is one of 2 distinct mammalian DNases. It hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. It has a key role in the degradation of nuclear DNA in cellular apoptosis during development [PMID:18812394]. Besieds, it is necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells. It has been demonstrated to be responsible forDNA degradation in apoptotic cells engulfed by macrophages and exogenous DNA on the skin surface [PMID:23019102]. DNASE2 exists some isoforms with MV 39 and 34 kDa. (PMID: 14644493)

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol; Angelica sinensis polysaccharides ameliorate 5-FU-induced stress anemia via promoting extramedullary erythroblastic island central macrophage-mediated erythroid differentiation; Authors - Biyao Wang
mouse	WB	J Biol Chem; Autolysosomal degradation of cytosolic chromatin fragments antagonizes oxidative stress-induced senescence.; Authors - Xiaojuan Han
human	WB	Pharmacol Res; Oroxylin A regulates cGAS DNA hypermethylation induced by methionine metabolism to promote HSC senescence; Authors - Danli Zhao
human,mouse	WB	Mol Nutr Food Res; Mangiferin protects DNase 2 abundance via Nrf2 activation to prevent cytosolic mtDNA accumulation during liver injury; Authors - Lisi Li
mouse	WB,IHC,IF	Transl Neurodegener; Neuronal double-stranded DNA accumulation induced by DNase II deficiency drives tau phosphorylation and neurodegeneration; Authors - Ling-Jie Li