DNAL1 Polyclonal antibody

DNAL1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 15809-1-AP

Synonyms

C14orf168, DNAL1, Dynein light chain 1, axonemal

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Validation Data Gallery

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  WB analysis using 15809-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15809-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15809-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15809-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15809-1-AP (DNAL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15809-1-AP

  IP result of anti-DNAL1 (IP:15809-1-AP, 4ug; Detection:15809-1-AP 1:500) with HeLa cells lysate 2600 ug.

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15809-1-AP targets DNAL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAL1 fusion protein Ag8545
• Full Name: dynein, axonemal, light chain 1
• Calculated Molecular Weight: 151 aa, 17 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC005343
• Gene Symbol: DNAL1
• Gene ID (NCBI): 83544
• RRID: AB_2095365
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAL1 was originally documented as the Chlamydomonas outer arm dynein axonemal light chain 1. DNAL1 has been documented to link components of the cytoskeleton with the molecular motor dynein, perhaps acting as a scaffold for larger functional structures (PMID: 22018492).