mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 17364-1-AP
IP result of anti-DNAJC5B (IP:17364-1-AP, 3ug; Detection:17364-1-AP 1:1500) with mouse testis tissue lysate 4000ug.
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PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
DNAJC5B encodes cysteine string proteins (CSP) beta. CSPs belong to the DnaJ-like chaperone family and play an important role in regulated exocytosis in neurons and endocrine cells. Mammals express three CSP proteins, α, β, γ. In contrast to the CSPα which is ubiquitously expressed, CSPβ is restricted to testis and auditory hair cell neurons. The predicted MW of DNAJC5B is around 22 kDa, while some modified forms exist (PMID: 17034881)
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
IP Figures
IP experiment of mouse testis using 17364-1-AP
IP result of anti-DNAJC5B (IP:17364-1-AP, 3ug; Detection:17364-1-AP 1:1500) with mouse testis tissue lysate 4000ug.
IF/ICC Figures
IF Staining of HepG2 using 17364-1-AP
Immunofluorescent analysis of HepG2 cells, using DNAJC5B antibody 17364-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
DNAJC5B Polyclonal antibody
Catalog Number
17364-1-AP
Citations
1
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IF/ICC : 1:10-1:100
Applications
WB, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat, pig
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
