DNAJC5B Polyclonal antibody

DNAJC5B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17364-1-AP

Synonyms

Cysteine-string protein isoform beta, CSP-beta, CSP beta, Beta cysteine string protein, Beta CSP

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Validation Data Gallery

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  WB analysis of mouse testis using 17364-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17364-1-AP (DNAJC5B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17364-1-AP

  IP result of anti-DNAJC5B (IP:17364-1-AP, 3ug; Detection:17364-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

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  IF Staining of HepG2 using 17364-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJC5B antibody 17364-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17364-1-AP targets DNAJC5B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC5B fusion protein Ag10744
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 5 beta
• Calculated Molecular Weight: 199 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC016742
• Gene Symbol: DNAJC5B
• Gene ID (NCBI): 85479
• RRID: AB_2095062
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC5B encodes cysteine string proteins (CSP) beta. CSPs belong to the DnaJ-like chaperone family and play an important role in regulated exocytosis in neurons and endocrine cells. Mammals express three CSP proteins, α, β, γ. In contrast to the CSPα which is ubiquitously expressed, CSPβ is restricted to testis and auditory hair cell neurons. The predicted MW of DNAJC5B is around 22 kDa, while some modified forms exist (PMID: 17034881)