DNAJC4 Polyclonal antibody

DNAJC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13853-1-AP

Synonyms

DANJC4, DnaJ like protein HSPF2, DNAJC4, HSPF2, MCG18

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 13853-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13853-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13853-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13853-1-AP (DNAJC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 13853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human breast cancer using 13853-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13853-1-AP (DNAJC4 antibody) at dilution of 1:100 (under 40x lens).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HepG2 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13853-1-AP targets DNAJC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC4 fusion protein Ag4826
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 4
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC044584
• Gene Symbol: DNAJC4
• Gene ID (NCBI): 3338
• RRID: AB_2095224
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.