DNAJC21 Monoclonal antibody, PBS Only

DNAJC21 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2F8A9

Cat no : 68343-1-PBS

Synonyms

DNAJA5, DNAJC21, GS3, JJJ1, Protein GS3

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Validation Data Gallery

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  WB analysis using 68343-1-Ig (same clone as 68343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68343-1-Ig (DNAJC21 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68343-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 68343-1-Ig (same clone as 68343-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC21 antibody (68343-1-Ig, Clone: 2F8A9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68343-1-PBS in a different storage buffer formulation.

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Product Information

68343-1-PBS targets DNAJC21 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJC21 fusion protein Ag20003
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 21
• Calculated Molecular Weight: 531 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC107577
• Gene Symbol: DNAJC21
• Gene ID (NCBI): 134218
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)