DNAJC21 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJC21 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 23411-1-AP

Synonyms

Protein GS3, GS3, DNAJA5

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Validation Data Gallery

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  WB analysis of HEK-293 using 23411-1-AP

  WB results of DNAJC21 antibody (23411-1-AP, 1:2000) with si-control and si-DNAJC21 transfected HEK293 cells.

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  WB analysis using 23411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 23411-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 23411-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 23411-1-AP (DNAJC21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 23411-1-AP

  IP result of anti-DNAJC21 (IP:23411-1-AP, 4ug; Detection:23411-1-AP 1:1000) with HEK-293 cells lysate 2700ug.

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  IHC staining of human pancreas using 23411-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 23411-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 23411-1-AP (DNAJC21 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 23411-1-AP

  Immunofluorescent analysis of HeLa cells using 23411-1-AP (DNAJC21 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, rat brain tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

23411-1-AP targets DNAJC21 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC21 fusion protein Ag20003
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 21
• Calculated Molecular Weight: 531 aa, 58 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC107577
• Gene Symbol: DNAJC21
• Gene ID (NCBI): 134218
• RRID: AB_2879274
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC21 is a ubiquitously expressed DnaJ (heat shock protein 40) chaperone protein. Recently DNAJC21 was reported to be implicated in rRNA biogenesis. Loss of DNAJC21 induced abnormal cell morphology and inhibited cell growth. Mutations in DNAJC21 cause bone marrow failure syndrome. (27346687)

Publications

Species	Application	Title
human	WB	Cell Stem Cell; HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis.; Authors - Kaosheng Lv
human	WB,IF	Am J Hum Genet; DNAJC21 Mutations Link a Cancer-Prone Bone Marrow Failure Syndrome to Corruption in 60S Ribosome Subunit Maturation.; Authors - Hemanth Tummala ; KD Validated