DNAJC11 Polyclonal antibody

DNAJC11 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 17331-1-AP

Synonyms

DNAJC11

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Validation Data Gallery

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  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17331-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, SH-SY5Y cells, HepG2 cells, mouse liver tissue
• Positive IHC detected in: human liver tissue, human skeletal muscle tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

17331-1-AP targets DNAJC11 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC11 fusion protein Ag11310
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 11
• Calculated Molecular Weight: 507 aa, 57 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC014145
• Gene Symbol: DNAJC11
• Gene ID (NCBI): 55735
• RRID: AB_2878385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.

Publications

Species	Application	Title
human	WB	J Proteome Res; Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.; Authors - Foteini Violitzi
mouse	WB	PLoS One; A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.; Authors - Fotis Ioakeimidis
human,mouse	WB	Nat Cell Biol; Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space; Authors - Elias Adriaenssens