DNAJC1 Polyclonal antibody

DNAJC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12362-1-AP

Synonyms

DnaJ protein homolog MTJ1, DNAJC1, DNAJL1, ERdj1, HTJ1, MTJ1

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Validation Data Gallery

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  WB analysis using 12362-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 12362-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12362-1-AP (DNAJC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 12362-1-AP

  IP result of anti-DNAJC1 (IP:12362-1-AP, 4ug; Detection:12362-1-AP 1:1000) with Raji cells lysate 3200 ug.

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  IHC staining of human breast cancer using 12362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12362-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12362-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12362-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12362-1-AP (DNAJC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells, Raji cells, NIH/3T3 cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12362-1-AP targets DNAJC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJC1 fusion protein Ag3030
• Full Name: DnaJ (Hsp40) homolog, subfamily C, member 1
• Calculated Molecular Weight: 554 aa, 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC030955
• Gene Symbol: DNAJC1
• Gene ID (NCBI): 64215
• RRID: AB_2094754
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Cancer Res Clin Oncol; DNAJC1 facilitates glioblastoma progression by promoting extracellular matrix reorganization and macrophage infiltration; Authors - Han Zhang