DNAJB9 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNAJB9 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13157-1-AP

Synonyms

DNAJB9, ERdj4, Mdg 1, MDG1, MST049, MSTP049

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Validation Data Gallery

• 

  WB analysis using 13157-1-AP

  Mouse kidney lysates were subjected to SDS PAGE followed by western blot with 13157-1-AP (DNAJB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13157-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13157-1-AP (DNAJB9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IP experiment of mouse testis using 13157-1-AP

  IP result of anti-DNAJB9 (IP:13157-1-AP, 4ug; Detection:13157-1-AP 1:500) with mouse testis tissue lysate 4800ug.

• 

  IHC staining of human breast cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 13157-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13157-1-AP (DNAJB9 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, mouse testis tissue
• Positive IP detected in: mouse testis tissue
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below

Product Information

13157-1-AP targets DNAJB9 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB9 fusion protein Ag3789
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 9
• Calculated Molecular Weight: 223 aa, 26 kDa
• Observed Molecular Weight: 26-30 kDa
• GenBank Accession Number: BC028912
• Gene Symbol: DNAJB9
• Gene ID (NCBI): 4189
• RRID: AB_2877918
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	J Pineal Res; Melatonin alleviates PM2.5 -induced glucose metabolism disorder and lipidome alteration by regulating endoplasmic reticulum stress; Authors - Zhou Du
human	WB	Mol Cell; Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.; Authors - Tsun-Kai Chang
human	WB	Elife; Cell-based HTS identifies a chemical chaperone for preventing ER protein aggregation and proteotoxicity.; Authors - Keisuke Kitakaze
human	WB	Hum Mol Genet; XBP1 variant 1 (Xv1) promotes mitosis of cancer cells involving upregulation of the polyglutamylase TTLL6.; Authors - Yongwang Zhong
human	IHC	Pathol Int; Early onset Congo red-positive fibrillary glomerulonephritis associated with glomerular DNAJB9 deposits mimicking renal amyloidosis; Authors - Mizue Goto
human	IHC	Case Rep Nephrol; Fibrillary Glomerulopathy with a High Level of Myeloperoxidase-ANCA: A Case Report.; Authors - Tomohiko Asakawa