DNAJB8 Polyclonal antibody

DNAJB8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA, IF

Conjugate

Unconjugated

Publications

3

Cat no : 17071-1-AP

Synonyms

DJ6, DNAJB8

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Validation Data Gallery

• 

  WB analysis of HeLa using 17071-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 2 publications below

Product Information

17071-1-AP targets DNAJB8 in WB, ELISA, IF applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB8 fusion protein Ag10899
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 8
• Calculated Molecular Weight: 232 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC029521
• Gene Symbol: DNAJB8
• Gene ID (NCBI): 165721
• RRID: AB_2094743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
mouse	WB,IF	STAR Protoc; Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.; Authors - Qianxing Zou
pig	WB	Vet Microbiol; The heat shock protein DNAJB8 inhibits pseudorabies virus replication by autophagy; Authors - Xiaoyong Chen