DNAJB7 Polyclonal antibody

DNAJB7 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 18540-1-AP

Synonyms

DJ5, DNAJB7, HSC3

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 18540-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 18540-1-AP (DNAJB7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 18540-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18540-1-AP (DNAJB7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 18540-1-AP is DNAJB7 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB7 fusion protein Ag13367
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 7
• Calculated Molecular Weight: 309 aa, 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC112135
• Gene Symbol: DNAJB7
• Gene ID (NCBI): 150353
• RRID: AB_2878550
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Reprod Biol Endocrinol; DNAJB7 is dispensable for male fertility in mice; Authors - Shun Bai