DNAJB14 Polyclonal antibody

DNAJB14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 16501-1-AP

Synonyms

UNQ9427/PRO34683, PRO34683, EGNR9427

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 16501-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16501-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 16501-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16501-1-AP

  IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug.

• 

  IHC staining of human ovary cancer using 16501-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 16501-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells, human testis tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 3 publications below

Product Information

16501-1-AP targets DNAJB14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DNAJB14 fusion protein Ag9668
• Full Name: DnaJ (Hsp40) homolog, subfamily B, member 14
• Calculated Molecular Weight: 379 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022248
• Gene Symbol: DNAJB14
• Gene ID (NCBI): 79982
• RRID: AB_2094414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Nat Commun; Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry.; Authors - Parikshit Bagchi
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
	WB	PLoS Pathog; A Non-enveloped Virus Hijacks Host Disaggregation Machinery to Translocate across the Endoplasmic Reticulum Membrane.; Authors - Madhu Sudhan Ravindran
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi
human	WB	J Virol; SGTA-dependent regulation of Hsc70 promotes cytosol entry of SV40 from the ER.; Authors - Allison Dupzyk
human	WB	J Virol; Ubqln4 facilitates ER-to-cytosol escape of a nonenveloped virus during infection.; Authors - Xiaofang Liu