DNAJA1 Monoclonal antibody

• Featured Product
• KD/KO Validated

DNAJA1 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgM

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B10A10

Cat no : 67184-1-Ig

Synonyms

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

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Validation Data Gallery

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  WB analysis using 67184-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67184-1-Ig

  WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

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  IF Staining of HepG2 using 67184-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MCF-7 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67184-1-Ig targets DNAJA1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgM
• Class: Monoclonal
• Type: Antibody
• Immunogen: DNAJA1 fusion protein Ag2284
• Full Name: DnaJ (Hsp40) homolog, subfamily A, member 1
• Calculated Molecular Weight: 397 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC008182
• Gene Symbol: DNAJA1
• Gene ID (NCBI): 3301
• RRID: AB_2882480
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Euglobulin precipitation
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.