DNA2 Polyclonal antibody

• Featured Product
• KD/KO Validated

DNA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18727-1-AP

Synonyms

KIAA0083, DNA2L

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Validation Data Gallery

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  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18727-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18727-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 18727-1-AP

  IP result of anti-DNA2 (IP:18727-1-AP, 4ug; Detection:18727-1-AP 1:300) with mouse liver tissue lysate 4800 ug.

• 

  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 18727-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18727-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA2 antibody (18727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse thymus tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

18727-1-AP targets DNA2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DNA replication helicase 2 homolog (yeast)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: NM_001080449
• Gene Symbol: DNA2
• Gene ID (NCBI): 1763
• RRID: AB_10637998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

Publications

Species	Application	Title
human	WB,IF	Nat Cell Biol; Stalled replication fork protection limits cGAS-STING and P-body-dependent innate immune signalling.; Authors - Ahmed Emam ; KD Validated
mouse	WB	Genes Dev; Abro1 maintains genome stability and limits replication stress by protecting replication fork stability.; Authors - Shengfeng Xu ; KO Validated
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Cell Cycle; The checkpoint clamp protein Rad9 facilitates DNA-end resection and prevents alternative non-homologous end joining.; Authors - Feng-Ling Tsai ; KD Validated
human	WB	Cells; Increased Resection at DSBs in G2-Phase Is a Unique Phenotype Associated with DNA-PKcs Defects That Is Not Shared by Other Factors of c-NHEJ.; Authors - Huaping Xiao ; KD Validated
human	IHC	Front Mol Biosci; Characteristic Analysis of Featured Genes Associated With Stemness Indices in Colorectal Cancer.; Authors - Yongqu Lu