DMXL2 Polyclonal antibody

DMXL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27123-1-AP

Synonyms

Dmx like 2, DmX like protein 2, DMXL2, KIAA0856, Rabconnectin 3, RC3

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Validation Data Gallery

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  WB analysis using 27123-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27123-1-AP (DMXL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 27123-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27123-1-AP (DMXL2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 27123-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 27123-1-AP (DMXL2 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse brain tissue
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27123-1-AP targets DMXL2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMXL2 fusion protein Ag25953
• Full Name: Dmx-like 2
• Calculated Molecular Weight: 3036 aa, 340 kDa
• Observed Molecular Weight: 340 kDa
• GenBank Accession Number: BC140781
• Gene Symbol: DMXL2
• Gene ID (NCBI): 23312
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.