DMXL2 Polyclonal antibody

DMXL2 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24415-1-AP

Synonyms

Dmx like 2, DmX like protein 2, DMXL2, KIAA0856, Rabconnectin 3, RC3

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Validation Data Gallery

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  IP experiment of mouse brain using 24415-1-AP

  IP result of anti-DMXL2 (IP:24415-1-AP, 4ug; Detection:24415-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human cerebellum using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24415-1-AP (DMXL2 antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24415-1-AP (DMXL2 antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cerebellum using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 24415-1-AP (DMXL2 Antibody) at dilution of 1:50.

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  IHC staining of human cerebellum using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 24415-1-AP (DMXL2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24415-1-AP (DMXL2 Antibody) at dilution of 1:50.

• 

  IHC staining of human kidney using 24415-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 24415-1-AP (DMXL2 Antibody) at dilution of 1:50.

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Tested Applications

• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human cerebellum tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

The immunogen of 24415-1-AP is DMXL2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DMXL2 fusion protein Ag19720
• Full Name: Dmx-like 2
• Calculated Molecular Weight: 3036 aa, 340 kDa
• GenBank Accession Number: BC140781
• Gene Symbol: DMXL2
• Gene ID (NCBI): 23312
• RRID: AB_2879534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles. In western blotting, we got 340 kDa and 104 kDa in mouse tissues. The two bands may represent different isoforms.

Publications

Species	Application	Title
human	WB	Brain; Biallelic DMXL2 mutations impair autophagy and cause Ohtahara syndrome with progressive course.; Authors - Alessandro Esposito
human	WB	Oncotarget; DMXL2 drives epithelial to mesenchymal transition in hormonal therapy resistant breast cancer through Notch hyper-activation.; Authors - Monica Faronato